1. western sandwich的解释

1. The sandwich ELISA was able to detect as low as 15.625pmolIml urinary AQP2 with 4.65% and 14.05% of intra-and inter-assay CVs. 3. Urinary AQP2 concentration by this assay was the same regularity as that by Western blot analysis in CHF model rats which were made by ligating the left coronary artery, and higher in large size MI group than in small size MI group.
    结果：1、直接ELISA法的待检尿液OD值非常低，与空白对照孔接近，无法使用。2、双抗体夹心ELISA法成功建立，灵敏度为15.625pmol/ml，批内及批间变异系数分别为4.65％和14.05％。3、用该法定量检测CHF模型大鼠42只，显示心力衰竭越重（心肌梗塞面积越大、梗塞后时间越长）及血钠越低，尿AQP2的表达量越多。

2. western sandwich是什么意思

2. Objective To investigate the therapeutic effect of Bushen Huoxue Tongluo Recipe on chronic aplastic anemia in old patients and to explore its mechanismMethods 67 old patients with CAA were randomly divided into treated group (34 cases) and control group (33 cases)The treated group was treated with BSHXTL recipe plus routine western medicine, while control group was treated with western medicine alone to examine respectively the apoptosis of bone marrow cells with enzymelinked double antibody sandwich immunospecific assaybefore and after treatmentResults The cure rate, remission, total effective rate of treated group were 4118%, 2352%, 8529% respectivelyThose of the control group were 2121%, 1819%, 6061% respectivelyThere was significant difference between the two groups in the therapeutic effect （P＜001）Apoptotic indexes in bone marrow cells in two groups before treatment were higher than that in healthy control group （P＜001）In treated group, apoptotic indexes in bone marrow cells after treatment were significantly lower than that before treatment （P＜001）, there was significant difference compared with that of control group（P＜001）Conclusions The therapeutic effect of BSHXTL recipe on old patients in CAA is reliable, and its side effect is smallThe therapeutic mechanism of BSHXTL recipe in treating old patients with CAA could inhibit overapoptosis of the bone marrow cells so as to promote bone marrow to rebuild hematopoietic function
    目的 观察补肾活血通络方治疗老年慢性再生障碍性贫血的临床疗效，探讨其作用机制。方法将67例老年CAA患者随机分为治疗组（34例）和对照组（33例）。治疗组用补肾活血通络方加常规西药治疗，对照组单用常规西药治疗。并分别于治疗前后采用酶联免疫吸附双抗体夹心法检测骨髓细胞凋亡状况。结果治疗组与对照组的基本治愈率、缓解率、总有效率分别为4118％、2352％、8529％和 2121％、1819％、6061％。两组疗效比较差异有显著性（P＜001）。治疗前两组骨髓细胞凋亡指数较健康对照组明显增高（P＜001），治疗后治疗组骨髓细胞凋亡指数较治疗前明显减低（P＜001），与对照组比较差异有显著性（P＜001）。结论补肾活血通络方治疗老年CAA疗效可靠、不良反应少。其作用机制可能是通过阻抑老年CAA患者骨髓造血细胞的过度凋亡，从而实现骨髓造血功能恢复重建。

3. Following counties are not included in the insurance coverage: In Asia: Afghanistan, Iraq, Cocos Islands, East Timor, British Indian Ocean Territories, Israel, Lebanon, Iran, Palestine, Sri Lanka In Africa: Eritrea, Rwanda, Somalia, Western Sahara, Saint Helena, Angola In the Pacific Rim: American Samoa, Bouvet Island, Christmas Island, French Southern Territories, Heard and McDonald, Kiribati, Marshall Islands, Micronesia, Nauru, Niue, Palau, Pitcairn, Solomon Islands, South Georgia and South Sandwich, Tokelau, Tonga, Tuvalu, US Minor outlying islands, Vanuatu, Wallis and Futuna.
    拒保国家和地区 The Countries and Territories where Assistance is not available 以下国家作拒保处理：亚洲：阿富汗，伊拉克，科科斯群岛，东帝汶，英属印度洋领地，以列，黎巴嫩，伊朗，巴勒斯坦，斯里兰卡；非洲：厄立特里亚，卢旺达，索马里，西撒哈拉，圣赫勒拿岛，安哥拉；大洋洲：美属萨摩亚群岛，布维岛，圣诞岛，属太平洋领地，赫德岛和麦克唐纳群岛，基里巴斯，马歇尔群岛，麦克罗尼西亚，瑙鲁，尼乌亚岛，巴伯儿图阿普群岛，皮特肯群岛，所罗门群岛，南乔治亚和南桑威治，托客劳群岛，汤加，图瓦卢，美国本土外小岛屿，瓦努阿图，沃利斯和富纳群岛；美洲：海地；南极洲：南极洲。

4. By using the McAbs, serum of angiostrongyliasis patient and sera of the rats infected with A. cantonensis were detected by Western blotting and double antibody sandwich ELISA respectively.
    使用所得的单克隆抗体以WESTERNBLOTTING检测广州管圆线虫病患者血清，并建立双抗体夹心ELISA法检测感染大鼠血清。