1. Hppe gene expression levels of iast/tet on/hppe cell strains were detected by rt pcr, immunocytochemistry and indirect immunofluorescence staining. results: a recombinant retroviral vector prevtre/hppe was constructed successfully.
结果：重组逆转录病毒载体prevtre/hppe构建成功；经挑选得到了高表达、低背景iast/tet on细胞株，其诱导倍数为20.6；在iast/tet on/hppe细胞株中，hppe基因表达受强力霉素调控，呈剂量依赖关系。
2. The X protein of Human hepatitis B virus acts as an indirect transcriptional transactivator to regulate the expression of many viral and cellular genes, as well as playing a critical role in pathogenesis and the development of Hepatocellular carcinoma.
王适群；吴建国摘要：乙肝病毒X蛋白作为一种间接的反式转录活化因子能调控许多细胞与病毒基因，在致病和肝细胞癌的发生发展中起着重要的作用。
3. The aim of this study was to illuminate the expression of proteins of the EGFR, IGF- I and IGF- I R in preimplantation bovine embryos by using indirect immunofluorescence and laser scanning confocal microscopy.
本研究利用免疫荧光和激光共聚焦扫描技术探讨了表皮生长因子受体，类胰岛素生长因子及其受体在牛早期胚胎中的蛋白表达。
4. indirect expression
4. Indirect immunofluorescence staining, confocal laser scanning microscopy and image analysis were used to study the expression of nephrin, podocin, α-actinin in glomeruli at day 1, 36hours, day 2, 5, 10, 15 and 20 after injections.
用间接免疫荧光染色、激光扫描共聚焦显微镜镜检及图像分析方法，检测PAN注射后36小时、2天、5天、10天、15天及20天大鼠肾小球中nephrin、podocin和α-actinin的蛋白表达量和分布。
5. By using indirect immunofluorescence and confocal laser scanning microscopy, the expression and localization of FPR in the primary glioma cells were detected.
目的观测甲酰化肽受体（formylpeptide receptor，FPR）在人恶性胶质瘤原代培养瘤细胞的表达和功能活性。
6. Then the expression of IGF-Ⅰ, IGF-ⅠR, IGF-Ⅱ and IGF-ⅡR in ovine oocytes and in vivo fertilized preimplantation embryos in different developmental stages were detected with indirect immunofluorescence staining, laser scanning confocol microscopy.
对小鼠和一些其他物种的研究发现植入前胚胎表达许多多肽类生长因子，同时胚胎表面亦检测到对应受体的存在。这些生长因子包括IGF、EGF、FGF、PDGF、TNF等。
7. indirect expression在线翻译
7. Indirect anaphora is a kind of linguistic phenomenon in which ananaphoric expression cannot find its corresponding antecedent in discourse.
间接回指是指语篇中回指成分无法在先前语篇中找到与其对应的先行成分的语言现象。
8. The indirect expression is a special way of the many kinds of performance.
间接表达是多种表现形式中的一种特殊形式。
9. In case of the potential communicative failure, the speaker makes efforts to design his indirect expression recognizable and understandable.
为了保证交际目的的实现，说话人需要使对方识别言语的间接形式并且理解言语的隐含意义。
10. There are several internationally recognized evaluation system development, we can choose as the vitality of the evaluation system, the expression for indirect Gross National Happiness.
国际上有几个公认的评价发展的体系，我们可以选择作为生命力的评价体系，用来间接表达国民幸福总值。
11. Using indirect immunofluorescence method, hHCA expression was examined in 36 patients with AML and 7 normal control subjects by flow cytometry.
方法应用间接免疫荧光法，流式细胞仪对36例急性髓性白血病患者和7名正常对照者进行检测。
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12. In short, Japanese people are inclined to use indirect expression for requests, but in refusal often use more direct expression.
简单而言，日本人倾向多用间接方式来请托，但若要拒绝时并不乏用直接表达。
13. They are carefully reviewed to provide a clear theoretical framework and tool for analyzing the conversational implicature and indirect expression.
本文从中国电视采访类节目中精选了大量的使用间接表达的对话，运用上述语用学理论对其会话含义进行了详细的分析。
14.
14. Methods: After culturing with different dose of Pentamidine for different time, cells were tested by MTT assay. Morphological changes was performed by light microscope. Apoptosis rate、cell cycle phase distribution and bcl-2 protein expression of Hela cells were measured by flow cytometry and indirect immunofluorescence technique of FCM.
应用MTT法检测不同浓度喷他脒对Hela细胞的生长抑制作用，光镜观察其形态学变化，流式细胞仪及流式细胞仪间接免疫荧光技术检测其细胞周期、细胞凋亡率及细胞内bcl-2蛋白的表达。
15. This expression may be used as an indirect testing method of noise equivalent bandpass.
该表达式可用作噪声等效带宽的一种间接测试方法。
16. The result of indirect immunifaction technique using FITC fluorescence tagging indicated that not only the expression of Bcl-2 protein decreased and the expression of Bax, Caspase-3 protein increased in Hela cells treated with dMChR(3μM, 30μM, 100μM)for 24 hours, but also the expression of Bcl-2 protein decreased and the expression of Bax, Caspase-3 protein increased in Hela cells treated with dMChR(30μM)for 12 hours, 24 hours. dMChR (100μM) and ChR(100μM) could decrease the expression of Bcl-2 protein and up-regulate of Bax, Caspase-3 protein in Hela cells, but the effect of dMChR was stronger that of ChR.
FITC荧光标记FCM分析结果显示：dMChR(3μM，30μM，100μM)处理Hela细胞24小时后，细胞的Bcl-2蛋白表达下调，Bax，Caspase-3蛋白表达上调，且呈浓度依赖性变化；同时100μM的dMChR下调Hela细胞Bcl-2蛋白表达和上调Hela细胞Bax，Caspase-3蛋白表达作用，较100μM的ChR强；dMChR(30μM)分别孵育Hela细胞12小时，24小时，细胞的Bcl-2蛋白表达下调，Bax，Caspase-3蛋白表达上调，呈时间依赖性变化。4。
17. This expression may be used as an indirect testing method of scan efficiency η.
该表达式也许可以作为扫描效率η的一种间接测试方法。
18. Our research was based on that nuclear factor κB is highly express in malignant glioma, and could direct or indirect control multi-angiogenesis factors''''expression, then to promote angiogenesis, use the gene engineering techniques truncate the phosphorylated situs in the special inhibitor of NFκB(mutant type IκBα) in the tumors. Upon stimulation, mutant type IκBα resisted to be phosphorylated and degrated, thus prevent the activation of NFκB and the expression of down stream genes, to continued and stably inhibit angiogenesis, then to prevent tumorigenesis and grow. And then, to discuss the multi mechanisms of signals''''conduction.
本研究利用核因子κB在恶性胶质瘤中高表达，且能够直接或间接调控多种促血管生长因子的表达，进而促进血管新生的特性，运用基因工程技术切除肿瘤细胞中核因子κB特异性抑制物IκBα基因片段中的磷酸化位点，使得肿瘤细胞受到刺激后IκBα无法进行磷酸化及随后的降解，不能释放核因子κB，无法促进下游目的基因的表达，从而达到持续稳定的抑制血管新生形成，进而抑制肿瘤生长、促进肿瘤细胞死亡的目的；同时，运用现代细胞分子生物学技术，进一步探讨并阐明此过程中的多种信号传导的分子机制。
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19. Hog cholera lapinised virus; E2 gene; Prokaryotic Expression; Indirect ELISA
猪瘟兔化弱毒； E2 基因；原核表达；间接 ELISA
20. Expression plasmids were transfected into 297T cells, the expression of fatl was detected by Western blot, and the distribution of this gene was analyzed by indirect immunofluorescense assay.
通过RT-PCR法扩增得到ω-3多聚不饱和脂肪酸脱氢酶基因fat1，构建重组真核表达载体pCMV-Myc-fat1，用脂质体法转染293T细胞，Western blot检测fat1的表达，并用间接免疫荧光确定其在293T细胞中的定位情况。