1. It was proved that the expressed proteins were insoluble and existed in the form of inclusion body. Primary purified protein was obtained.
表达产物以不溶性包涵体形式存在，此包涵体蛋白不易降解，经处理得到初步纯化的蛋白质。
2. Both the expressed proteins were insoluble aggregates, which reached 80% purity after rapid preparation of the products as the inclusion body.
表达产物经包含体制备达到80％以上纯度。N端序列测定的15个氨基酸，与重组cDNA基因编码的序列相同。
3. inclusion body的近义词
3. In the research of muscular dystrophies and inclusion-body myopathies, several overseas authors have found that nitricoxide synthase and oxidative stress can induce skeletal muscle atrophy, and there is the correlation between them.
NOS的活性在CAM中是否有变化，与CAM肌萎缩的发生有无相关性，NOS引起的骨胳肌氧化-抗氧化平衡失调是否为发病机理中的重要环节，国内外均尚未见报道。
4. inclusion body的翻译
4. How to refold inclusion body proteins with many pairs of disulfide bonds into natural conformation with high efficiency is a challengeable problem at present, and it is becoming one of the industrial bottlenecks in bioengineering field.
如何对富含二硫键的包涵体蛋白进行高效的体外复性使其获得正确的空间结构是重组蛋白生产所面临的巨大挑战，现已成为制约生物工程技术产业化的瓶颈问题之一。
5. But 5-helix was apt to form inclusion body when expressed directly in prokaryotic cell and was difficult to renature, which causes inconvenience to future study.
但5-helix基因在原核细胞中直接表达时易形成包涵体，复性困难，给研究带来不便。
6.
6. RESULTS: Among 119 patients with IM, there were 11 patients with severe necrosis, 19 patients with moderate necrosis, 27 patients with mild necrosis, 20 patients with perifascicular necrotic/atrophic myositis, 22 patients with non-inflammatory cellular infiltrative necrotic myositis, 11 patients with interstitial myositis, 3 patients with muscular fasciitis, 4 patients with inclusion-body myositis, one patients with granulomatous myositis, and one patients with proliferous myositis.
结果：共检出IM119例，其中重、中、轻度坏死性肌炎分别为11例、19例和27例，束周坏死/萎缩性肌炎20例，无炎细胞浸润的坏死性肌炎22例，间质性肌炎11例，肌筋膜炎3例，包涵体肌炎4例，肉芽肿性肌炎和增殖性肌炎各1例。
7. The expressed prodict existed in a form of inclusion body whose relative molecular mass was about 19KD, and contained about 30% of total somatic protein or 80% of total inclusion body protein. Purity of chIFN-α was more than 95% after Ni(superscript 2+)-NTA purification.
工程菌诱导表达后的电泳图谱在相对分子量约19kDa的位置出现明显目的条带，约占菌体总蛋白的30%，表达产物主要以包涵体形式存在，经过NI(上标 2+)-NTA亲和层析纯化，SDS-PAGE电泳后经凝胶扫描纯度达95%以上。
8. The scFv formed inclusion body in E. Coli. 8 mol/L urea was used to solubilize the inclusion body, and the scFv was then purified in two steps, including Ni 2+ chelating affinity chromatography and gel filtration.
大肠杆菌表达的单链抗体包涵体，用8 mol/L尿素裂解，经过Ni离子螯合亲和层析和凝胶过滤两步纯化后，纯度达到97%以上，再通过透析复性除去尿素。
9. The way of preparing mouse Nanog fusion protein from the inclusion body was established, which was prepared for activation detection and mechanism of gene expression and regulation.
建立了一种可行的从包涵体中纯化重组小鼠Nanog蛋白的方法，为Nanog蛋白的活性检测及其基因表达调控机理的功能研究奠定基础。
10. Pieris rapae Granulosis Viruses strain Jing Nong 801 (PrGV-JN 801) were purified by sucrose-gradient centrifugation and then dissolved in sodium carbonate. The dissolved inclusion-body protein which was isolated and purified from the dissolution mixture by using Sepharose 2B column chromatography showed only one protein band by SDS-PAGE.
菜粉蝶颗粒体病毒京农801株(PrGV-JN801)，经蔗糖梯度离心纯化、碳酸钠溶解后，再经Sepharose 2B柱层析分离纯化，纯化的PrGV-JN801包涵体蛋白，在SDS-PAGE上呈1条带。
11. Results The inclusion body expression product accounted for 24.4% of total bacterial protein. The purity of recombinant protein was about 95% after purfication. The mean size of folate liposome was 0.7 ＋ 0.4 μm. Protective rates of PBS alone, and liposome alone all were 0, While in growps of Kat plus CT, liposome- encapsulated Kat and liposome - encapsulated Kat plus CT were 73.3%, 66.7% and 86.7% respectively.
PBS组和空白脂质体组保护率均为0，而Kat重组蛋白＋CT组、脂质体包裹Kat重组蛋白组、脂质体包裹Kat重组蛋白＋CT组的保护率分别为73.3％、66.7％和86.7％，且均能使免疫小鼠胃黏膜H·pylori感染数目明显减少，三个疫苗组淋巴细胞增殖试验均为阳性。
12.
12. The expression of protein showed that sorcin was only overexpressed in Escherichia coli BL21 and Rosetta-BL21 as inclusion body.
并且，类sorcin蛋白在Rosetta-BL21菌株中的表达量要比在BL21菌株中大。
13. The vector was transformed into Escherichia coli i BL21(DE3). The fusion protein was expressed successfully in the form of inclusion body. The recombination protein of IL-1ra-Fce was highly purified by chromatography of gel filtration and ion exchange, which was identifited by Western blotting. The cell assay showed that the activity of IL-1ra-Fce was as high as IL-1ra in vitro after refolding. The pharmacokenetic profile of IL-1ra-Fcε and IL-1ra was analyzed, and the half time of IL-1ra-Fce is 4.78 times than that of IL-1ra.
将其转化大肠杆菌BL21(DE3)，实现了融合蛋白的高效表达，Western blotting结果表明表达蛋白为目的融合蛋白，主要以包涵体形式存在；利用分子筛和阳离子交换层析对表达产物经进行了纯化，纯化的包涵体复性后经体外功能试验表明，融合蛋白的活性与IL-1ra没有显著性差异；初步药代动力学分析显示IL-1ra-Fce半衰期比IL-1ra延长了4.78倍。
14. The research aimed to observe the inclusion body in pyramidale cytoplasm in hippocampus of cerebral cortex in shepherd dog with rabies.
观察患狂犬病的牧羊犬大脑皮层海马的锥体细胞浆内的包涵体。
15. Parasitizing specially in the cytoplasma of small granular hemocytes, RLOs formed an inclusion body in host cell, in which, RLOs propagated in mass and caused the host cell swelling and disorganizing, subsequently released and infected other target histocytes, resulted in the arising of such symptoms as debility and shivering of limbs, etc..
RLOs专一性寄生于小颗粒细胞的胞质内，以包涵体的形式生长繁殖，随着RLOs的快速增殖，包涵体内RLOs数目显著增多，且多呈圆形，包涵体极度膨胀，将细胞核压向周边，最终细胞被胀破，释放出RLOs再去侵染其它组织和器官，造成颤抖、肌无力等全身症状。
16. inclusion body的近义词
16. After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant GST2E0 protein by GST affinity columns.
使用分步透析法对变性的包涵体进行复性，将复性蛋白过GST亲和层析柱得到纯化的GST E0融合蛋白。
17. Disclosed are absorbent cores for inclusion in articles such as diapers, incontinent briefs, training pants, diaper holders and liners, feminine hygiene garments, and the like, designed to provide improved fit and comfort for the wearer while adequately containing body exudates.
本发明披露了一种包含在制品中的吸收芯，所述制品如尿布，失禁用三角裤，长内裤，尿布托和衬垫，妇女卫生保健服装等所述制品被设计成给穿戴提供改进的配合性以及舒适性；同时能包容足够的身体排出物。
18. Participants will go through a process exploring inclusion, participation, storytelling, and drama of touch; decolonizing the mindful body; empathizing with others.
参与者将经历一连串的探索过程：共融、参与、说故事、触摸戏剧、释放身体，与别人建立同理心。
19. Combined with SDSPAGE of recombinant protein, the authorspres umed that the peak absorption at～1630 cm-1 is most likey to beassigned to protein aggregate within inclusion body.
进一步对酰胺栺带区域进行傅里叶去卷积处理，结合高斯曲线拟合，发现在Sc样品中随着诱导时间的增加，聚集体含量逐渐增加，这与SDS-PAGE结果一致。
20. Purpose to study a technology of prepared recombinant human angiostatin from inclusion body.
目的：研究从包涵体中制备重组人血管抑素的可行工艺。