1. Immunohistofluorescent staining was used to identify the binding specificity of GEBP11 in gastric cancer tissues. 5. GEBP11 was labeled with ~(99)Tc~mO_4~- using direct labeling method, and was labeled with ~(131)I using NBS labeling method. Then the labeled peptides were validated for radiochemical yield, specific activity, and in vitro. stability. 6. The bioactivity of ~(99)Tc~m-GEBP11 or ~(131)I-GEBP11 was validated by cell receptor autoradiography in cultured HUVECs. 7. Receptor binding assay was performed in vitro. to analyze quantitatively the binding specificity and affinity of GEBP11 to HUVEC, the receptor intensity on HUVEC, and the difference of affinity of GEBP11 binding to Co-HUVEC and HUVEC. 8. The radioactivity of all organs of nude mice injected with ~(131)I-GEBP11 was determined and the radioactivity of all organs per g was calculated to analyze the specific distribution of ~(131)I-GEBP11.9. Gamma camera images of nude mice bearing tumor xenografts of human gastric carcinoma injected with ~(99)Tc~m-GEBP11 was obtained 0.5-24 h after being injected to identify the targeting ability of labeled peptide to tumor tissues. 10. MTT assay on HUVECs and antitumor assay in nude mice bearing tumor xenografts of human gastric cancer were performed to evaluate the effects of ~(131)I-GEBP11 on HUVECs and xenografts. 11. Immunohistochemical staining was used for MVD counting in tumor. 12. H. E. staining, blood cells analysis and blood biochemical indicator analysis were performed to investigate the injury of liver and bone marrow. 13. Tube formation assay in matrigel, CAM angiogenesis assay and angionenesis induced by matrigel in mice were performed to identify the effects of GEBP11 on angiogenesis.
1、通过蓝色噬斑形成实验及GEBP11、IN11噬菌体在荷瘤裸鼠体内的竞争抑制实验检测噬菌体体内归巢的特异性；2、免疫荧光技术检测GEBP11在荷瘤裸鼠体内的结合特异性；3、免疫细胞化学或荧光检测GEBP11在Co-HUVECs中的定位及内化；4、免疫组织荧光检测GEBP11在人胃癌组织中的结合特异性；5、采用直接法~(99)Tc~mO_4~-标记GEBP11，采用NBS法~(131)I标记GEBP11，纸层析法测定标记率、放射化学纯度等；6、放射自显影鉴定GEBP11同位素探针的结合活性；7、受体放射配基结合分析实验鉴定受体亲和力及受体细胞密度；8、同位素示踪技术检测~(131)I-GEBP11在荷瘤裸鼠体内的生物学分布；9、SPECT成像技术进行荷人胃癌裸鼠体内~(99)Tc~m-GEBP11显像；10、MTT细胞增殖实验、荷瘤鼠抑瘤实验评价~(131)I-GEBP11对内皮细胞及荷瘤鼠肿瘤的抑制能力；11、免疫组织化学染色计数瘤组织MVD；12、病理学HE染色、血液血细胞分析及生化指标检测观察肝脏损伤及骨髓抑制情况；13、Matrigel管状结构形成实验、鸡胚绒毛尿囊膜血管生成抑制实验、小鼠体内Matrigel Plug诱导血管生成实验分析GEBP11对血管生成的影响；14、MTT细胞增殖实验、细胞周期及凋亡分析、细胞侵袭迁移实验、细胞粘附实验探讨GEBP11抑制血管生成的细胞机制；15、基因表达谱芯片技术筛选GEBP11短肽作用Co-HUVECs后的差异表达基因。
2. As ice-covered areas open up, the Arctic will experience more human activity than ever before.
因为随着冰川区域的开发，北极将会经历前所未有的人类活动。
3. 公共查询·英语单词
3. Religion is a universal phenomenon extending as far back in history as we can trace human activity and showed among people in every part of the world.
棕教是一种普遍现象，历史上可以回溯到我们可追溯的有人类活动的时期，并在世界每一部分蹦民族中都有。
4. The leaders will also release a final statement that is expected to say human activity causes some climate change.
领导人们也将发表一个共同声明，强调人类的活动引起了气候的变化。
5. It shows that runoff is closely correlated with the features of individual precipitation, and it is also affected by vegetation within a short period of time. Raindrop erosion, sheet flow erosion and rill erosion on the slope are the main soil erosion patterns; sediment yield occurs mainly in flood season. Human activity and individual precipitation are two important factors that control the soil erosion at a small watershed. Comprehensive management on the slope in a small watershed is an efficient measure of water and soil conservation.
分析表明，小流域的产流方式容易随次降雨条件的变化而变化，植被因素在较小时间尺度内就会影响产流；产沙主要来源于坡面上的溅蚀、片蚀和细沟侵蚀，时间主要集中在汛期；人类活动以及次降雨条件是控制小流域侵蚀产沙的重要因素；在小流域内进行坡面的综合治理是水土保持的有效措施。
6. Objective To construct enhanced green fluorescence protein report gene expression vector under the control of human secretory leukoprotease inhibitor gene promoter and to test the activity of this promoter in the non-small cell lung cancer cell lines.
目的 构建SLPI基因启动子驱动下EGFP表达载体并验证该启动子在非小细胞肺癌细胞株中的特异调控活性。
7. human activity的反义词
7. The human does not leave, and in the activity, the switch continues the breakover; After the human leaves, the switch time delay automatic shut-off load, is bright the human to the lamp, the human extinguishes to the lamp, kind convenient, safe energy conservation.
人不离开且在活动，开关持续导通；人离开后，开关延时自动关闭负载，人到灯亮，人离灯熄，亲切方便，安全节能。
8. It is the base of settling down and getting on with one''s work, and a principle of the human communication, is a foundation of the economic activity, it is the tactics of fathing country.
诚信是安身立命的根本，是人际交往的原则，是经济活动的基础，也是治理国家的要略。
9. human activity的意思
9. The agglutination for rabbit was the highest among erythrocytes of human, rabbit, common carp and crucian carp. The hemagglutinating activity for rabbit could not be inhibited by D-fructose, D-mannose, D-galactose, glucose, sucrose, manrmn, gamma-globulin and egg albumin, but inhibited by bovine-thyroglobulin, and the minimum inhibitory concentration was 3.10 mg/ml.
该凝集素可以凝集人的A、B、AB、O型红细胞，且凝集活性相同，在对人、兔、鲤、鲫的红细胞的凝集作用中，对兔红细胞的凝集作用最强，且不被己测试的D-半乳糖、D-果糖、D-甘露糖、葡萄糖、蔗糖、甘露聚糖、γ-球蛋白、卵清蛋白所抑制，仅被牛甲状腺球蛋白抑制，最小抑制质量浪度为3.10mg/ml。
10. Objective To observed the effects of 5 hydroxyl group of genistein on recombinant human protein kinase CK2 holoenzyme activity.
目的 观察染料木黄酮C5位上的羟基对其抑制重组人蛋白激酶CK2全酶活性的影响。
11. This study was designed to investigate the effect of selenium dioxide(SeO_2) on human cervical cancer cell line, in culture, to describe the change of Hela cell''s growth, proliferation and telomerase activity, moreover, to reveal the relation of cervical cancer cell''s proliferation and telomerase activity.
本研究旨在通过观察体外培养的宫颈癌细胞株HeLa细胞在二氧化硒(selenium dioxide，SeO_2)的作用下，HeLa细胞生长增殖的变化及端粒酶活性的变化，探讨宫颈癌细胞的增殖与端粒酶活性之间的关系。
12. English education must realize to the education for all-around development transformation, wants the big area to train the talented person who the high quality will use English human relations, but the classroom instruction will be in the school one item is stablest, the holding time to be frequently most, the design area to be biggest, the content most widespread activity.
英语教育要实现向素质教育的转变，就要大面积培养高素质的会用英语交际的人才，而课堂教学是学校中一项最稳定、最经常、占用时间最多、设计面积最大、内容最广泛的活动。事实上优秀的课堂教学完全8 t tt8。
13.
13. The environment is the foundation of activity of human material production.
环境是人类物质生产活动的基础。
14. With the rapid development of the society, more and more attention has been paid to the influence of human activity on environment and food pollution.
随着社会的发展，人们越来越关心人类活动对环境和食品污染造成的影响。
15. And land desertication develops as a result of the comprehensive effects of environment and human activity.
土地沙漠化是自然与人为因素共同作用的结果，以过牧为主导因素。
16. Educational life should be a harmonious process of unifying human activity and environment.
教育生活应当是活动与环境和谐统一的过程。
17. Mr. Li did research on nerve cell activity in mice that could help improve understanding of the human brain.
李先生对老鼠神经细胞的活动进行了研究，这对人脑的研究也有帮助。
18. Black soil was one of the highest organic matters in china, after reclamation organic matter lost rapidly because of human activity.
黑土是中国土壤有机质含量较高的土类之一，开垦以后由于人类活动的影响黑土有机质大量损失。
19. In order to understand the change tendency of TRAP in serum of normal human beings, we systematically determined the serum levels of TRAP activity in a group of 237 normal persons aged 0.5-72 years divided into different decades, using P-nitrophenyl phosphate disodium salt as substrate.
本文为观察TRAP活性在正常人血清中动态变化趋势，采用对硝基酚磷酸盐法测定了237例(年龄0.5～72岁，平均年龄35.3±18.0)不同年龄段(每隔10岁为一年龄段)正常人血清TRAP活性。
20. human activity
20. As the development of human society, population pressure is strengthened ceaselessly and unreasonable activity, be like excessive depasture, excessive cultivate, chaos is collected dig etc, make contemporary prairie happens generally degrade.
随着人类社会的发展，人口压力不断加强以及不合理的活动，如过度放牧、滥垦、乱采挖等，使现代草原普遍发生退化。
1. It is essential that we protect the environment and ecosystem which has been seriously damaged in some areas by unnecessary human activity.
2. Inner Mongolia has many natural deserts and such careless human activity has given them a perfect opportunity to expand - and expand they have.
3. He also agrees that the level of it is rising because of human activity and that this should warm the climate.
4. The series of IPCC reports made a big dent in the credibility of lingering skepticism about the link between human activity and global warming.
5. The Defense Intelligence Agency''s human intelligence collection activity would become a separate agency, like the former CIA directorate of operations.
6. Understanding how human activity modifies the atmosphere and oceans governing our planet''s weather is enormously difficult.
7. While Hanwang''s old town has been sealed off since rescue work ended, there are fleeting clues of human activity.
8. It is the site of some of the earliest traces of human activity in that area.
9. China''s own National Assessment Report concluded in 2005 that climate change is taking place, and that it is most likely due to human activity.
10. The latest scientific reports have shown it is happening and that human activity is responsible.