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1. When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added, kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.
    选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

2. 公共查询·英语单词大全

2. Will be launched ahead bucket, so I fight down for dumping.
    将铲斗向前推出，使斗口朝下进行卸土。

3. A rotary drill digging squeeze engine, it consists of the body and control systems, masts, winches, the up and down support package, squeezed oil tank, drill pipe, dynamic head, spin arm and spin bucket.
    一种旋挖挤扩钻机，它是由车身及控制系统、桅杆、卷扬、上下支撑套、挤扩油缸、钻杆、动力头、旋扩臂和旋挖斗组成的。

4. She let down the bucket into the well.
    她把吊桶入下井里。

5. bucket down在线翻译

5. Place the bucket under the trap and loosen the connection to the portion of the pipe leading down from the sink to the trap.
    把水桶下圈套，松动的连接管部分领导下来沉陷阱。

6. Select the paint bucket tool, in the top tool bar switch to pattern, then select your pattern in the drop down box.
    选择油漆桶工具，在顶部的工具栏里转到图案，然后在菜单里选择你的图案。

7. Within a few days, however, he would be deflated again, more stooped than ever, just like a fish bought live in the street and put in a bucket of water, where after thrashing about for a while it presently quiets down.
    精神了两三天，夏先生又不大出气了，而且腰弯得更深了些，很象由街上买来的活鱼，乍放在水中欢炽一会儿，不久便又老实了。

8. He came down the hill carrying a bucket of water.
    他提著一桶水下山来了。

9. When the device has just started, the air inside the tubes and the low temperature inside the condensate into the steam trap, inverted bucket by its own weight to fall, driven down bucket link lever to open the valve heart valve, air and cold condensate discharge rapidly.
    当装置刚启动时，管道内的空气和低温凝结水进入疏水阀内，倒吊桶靠自身重量下坠，倒吊桶连接杠杆带动阀心开启阀门，空气和低温凝结水迅速排出。

10.

10. Pour a bucket on the opening hole, when a part of the steam discharged from the hole, the other part of the steam condensate produced, inverted bucket to lose buoyancy, by its own weight goes down, inverted bucket link lever to open the valve spool driven, cycle work, intermittent drainage.
    倒吊桶上开有一小孔，当一部份蒸汽从小孔排出，另一部份蒸汽产生凝结水，倒吊桶失去浮力，靠自身重量向下沉，倒吊桶连接杠杆带动阀芯开启阀门，循环工作，间断排水。

11. Wagner does not know where to put it out of the bucket of cold water, standing behind Dante and fell on the straight Leng Leng down...
      瓦格纳也不知道从哪搞来了一桶凉水，站在但丁的背后就直愣愣地倒了下去。。。。

12. The operation process is as follows. Firstly steel ball run down from charging bucket.
      视觉检测系统开发的主要模块为图像采集与处理，图像分割，图像识别与匹配、执行检测。

13. Free float steam trap is only half of a semi-float type of ball bucket for moving parts opening down the ball bucket that is open and close cases but also seals.
      ziyou半浮球式疏水阀只有一个半浮球式的球桶为活动部件，闭口朝下，球桶即是启闭件，又是密封件。

14. 公共查询·英语单词

14. Free half-float steam trap: Free float steam trap is only half of a semi-float type of ball bucket for moving parts, opening down, the ball bucket that is open and close cases, but also seals.
      自由半浮球式疏水阀：自由半浮球式疏水阀只有一个半浮球式的球桶为活动部件，开口朝下，球桶即是启闭件，又是密封件。

15. PC navar sends out the clear disheartened signal, outlet, the be in charge of the pneumatic plate valve and upper ball check closes voluntarily, the pulse valve opens while resistance rises to certain numerical value, pulse air current distribution implement nitrogen gas enters, filters a bag as a result of gushing a blowpipe gushing from bag mouth to dilate rapidly, the dust filtering bag outside outside is shaken off down, drop-in lower part cone-shape ash bucket, the valve gushes a final pulse boasting being over, the dust catcher continues keeping idle state, Let thinner tube body inner dust have a motionless settlement process.
      当阻力上升到一定数值时，微机控制系统发出清灰信号，出口支管气动碟阀和上球阀自动关闭，脉冲阀开启，脉冲气流分布器氮气经喷吹管从袋口喷入，滤袋急速扩张，滤袋外表面的粉尘被抖落下来，落入下部锥形灰斗，最后一个脉冲阀喷吹结束，除尘器继续保持静止状态，使筒体内较细的粉尘有一个静止沉降的过程。

16. Our grab nickel production, nickel is mainly used to crawl Hard fine powder, red clay minerals such as highly viscous due to the recent nickel Hard fine powder, red clay and other minerals to be widely used, so Many major ports on the existence of such a problem, in which the ore handling when loading and unloading there is a low efficiency, has been a lot of ore grab adhesive wall, our company in order to solve this dilemma has been studied and produced by such a professional crawl very sticky ore grab, the principle is: grab the lowbeam calculated to increase the adoption of two pieces of solder, including the specific location of board and grab the bucket to create a circular flap, so Open grab discharging, the fight is on board, including the body wall from the top down (no contact with body panels Doo), to stick the body in fighting the viscous wall down, including mine, so, which greatly improved the handling grab the efficiency of ports and factories to solve the problem, which is purpuratus grab a patent, other manufacturers can not be produced.
      我公司生产的镍矿抓斗，主要用来抓取镍矿、铁精粉、红粘土等粘性很强的矿石，由于近来镍矿、铁精粉、红粘土等矿石在被人们广泛使用，因此，许多大港口就存在着这样一个问题，在装卸这此矿石的时候，存在着装卸效率低，抓斗内壁被很多矿石粘着，我公司为了解决这一难题，现已研究并生产出来这种专业抓取很粘的矿石抓斗，原理是：在抓斗的下承梁上通过计算得出要加焊俩块括板的具体位置，并要把抓斗的斗瓣做成圆形，这样在抓斗打开卸料时，括板就在斗体内壁从上而下，把粘在斗体内壁的粘矿括了下来，所以，这就大大提高了抓斗的装卸效率，为港口和工厂解决了这一难题，这种抓斗是紫石的一项专利，其它厂家是无法生产出来的。

17. bucket down

17. Our grab nickel production, nickel is mainly used to crawl, 铁精粉, red clay minerals such as highly viscous due to the recent nickel 铁精粉, red clay and other minerals to be widely used, so Many major ports on the existence of such a problem, in which the ore handling when loading and unloading there is a low efficiency, has been a lot of ore grab adhesive wall, our company in order to solve this dilemma has been studied and produced by such a professional crawl very sticky ore grab, the principle is: grab the next 承梁 calculated to increase the adoption of two pieces of solder, including the specific location of board and grab the bucket to create a circular flap, so Open grab discharging, the fight is on board, including the body wall from the top down (no contact with body panels Doo), to stick the body in fighting the viscous wall down, including mine, so, which greatly improved the handling grab the efficiency of ports and factories to solve the problem, which is purpuratus grab a patent, other manufacturers can not be produced.
      我公司生产的镍矿抓斗，主要用来抓取镍矿、铁精粉、红粘土等粘性很强的矿石，由于近来镍矿、铁精粉、红粘土等矿石在被人们广泛使用，因此，许多大港口就存在着这样一个问题，在装卸这此矿石的时候，存在着装卸效率低，抓斗内壁被很多矿石粘着，我公司为了解决这一难题，现已研究并生产出来这种专业抓取很粘的矿石抓斗，原理是：在抓斗的下承梁上通过计算得出要加焊俩块括板的具体位置，并要把抓斗的斗瓣做成圆形，这样在抓斗打开卸料时，括板就在斗体内壁从上而下，把粘在斗体内壁的粘矿括了下来，所以，这就大大提高了抓斗的装卸效率，为港口和工厂解决了这一难题，这种抓斗是紫石的一项专利，其它厂家是无法生产出来的。

18. Our grab nickel production, nickel is mainly used to crawl, 铁精粉, red clay minerals such as highly viscous due to the recent nickel铁精粉, red clayother minerals to be widely used, so Many major ports on the existence of such a problem, in which the ore handling when loadingunloading there is a low efficiency, has been a lot of ore grab adhesive wall, our company in order to solve this dilemma has been studiedproduced by such a professional crawl very sticky ore grab, the principle is: grab the next承梁calculated to increase the adoption of two pieces of solder, including the specific location of boardgrab the bucket to a circular flap, so Open grab discharging, the fight is on board, including the body wall from the top down (no contact with body panels Doo), to stick the body in fighting the viscous wall down, including mine, so, which greatly improved the handling grab the efficiency of portsfactories to solve the problem, which is purpuratus grab a patent, other manufacturers can not be produced.
      我公司生产的镍矿抓斗，主要用来抓取镍矿、铁精粉、红粘土等粘性很强的矿石，由于近来镍矿、铁精粉、红粘土等矿石在被人们广泛使用，因此，许多大港口就存在着这样一个问题，在装卸这此矿石的时候，存在着装卸效率低，抓斗内壁被很多矿石粘着，我公司为了解决这一难题，现已研究并生产出来这种专业抓取很粘的矿石抓斗，原理是：在抓斗的下承梁上通过计算得出要加焊俩块括板的具体位置，并要把抓斗的斗瓣做成圆形，这样在抓斗打开卸料时，括板就在斗体内壁从上而下，把粘在斗体内壁的粘矿括了下来，所以，这就大大提高了抓斗的装卸效率，为港口和工厂解决了这一难题，这种抓斗是紫石的一项专利，其它厂家是无法生产出来的。

19. Most nights in the Bucket home, dinner is a watered-down bowl of cabbage soup, which young Charlie gladly shares with his mother and father and both pairs of grandparents.
      在Bucket家中的大多数的夜晚，晚餐是一碗冲淡了的卷心菜汤，年轻的查理高兴地和他的母亲、父亲、祖父还有祖母一起分享。

20. He sat down on a bucket of deep-fryer oil.
      他坐在一个油桶上。