1. Methods Slit-lamp examination for specific lens opacities was taken by the opthalmic doctor, and in twenty-five patients of three pedigree with DM, CTG trinulceotide repeat numbers located in the 3, untraslated region of MTPK on chromosome 19q13.2-3 were analyzed by using long Expand TM Template PCR system.
裂隙灯检查受检者晶状体情况,长模板扩增TMPCR法检测19q13.2-3位点上蛋白激酶基因3,端非翻译区上CTG三核苷酸重复次数,并比较白内障与CTG重复次数的关系。
2. The subendocardial and sub-epicardial tissues were separated from the mid-layer region with opthalmic scissors. Single cells in each region were obtained by gentle shaking and dispersing in a chamber filled with normal Tyrode''s solution. The whole-cell patch clamp technique was used to record the current while change the composition of the perfusate.
采用胶原酶二步消化法分离兔心肌细胞,用锐利眼科剪分离左室游离壁内、中、外三层心肌,改变灌流液的成份,采用全细胞膜片钳记录离子电流。
3. Results It was found that in dogs the opthalmic artery originated from the maxillary artery, and that its root formed an angle with infraorbital artery.
结果在犬的解剖中发现眼动脉发自上颌动脉,起点处与眶下动脉成夹角。