1. Draining lymph nodes isolated from the BALB/C mice immunized with rabbit skin collagen Type Ⅲ accord- ing to a rapid immunization protocol, the lympho- cytes were fused with the myeloma cell line NS-1, 3 out of total of 201 growing hybridomas we obtained were producing collagen Type Ⅲ reactive antibodies after 3 limited dilution clones performed.
本实验采用快速免疫的方法用兔皮肤Ⅲ型胶原免疫BLAB/C小鼠,取淋巴结的细胞与小鼠骨髓瘤细胞NS-1融合,240孔中201孔有杂交瘤生长,用ELISA检测有6孔反应阳性。选3孔生长良好,抗体浓度高的细胞进行有效的有限稀释法克隆3次,获3株分泌抗Ⅲ型胶原单克隆抗体的杂交瘤细胞。其中二株属IgG2b,一株为IgG1。
2. If you want some bad luck, I''ll blab it out, but if you wanna be safe
如果你想要霉运的话,我会说出来的,但是如果你们想要安全点儿的话
3. It''s a secret and I''m not going to have you blab it out.
这是个秘密,不许你说出去。
4. He will be sure to blab our secret out, and it will be all over the town in no time
他一定会把我们的秘密泄漏出去,全城的人马上都会知道这件事。
5. You want some bad luck, I''ll blab it out.
你想交霉运,我就讲出来
6. You want some bad luck, I''ll blab it out, but if you wanna be safe, I''ll keep my mouth shut.
如果你想要霉运的话,我会说出来的,但是如果你们想要安全点儿的话,我一定不会说的。
7. When you force an issue, you blab without having thought out what you need to say, which increases the chance that you`ll both blurt out things you`ll regret later, says Piver.
当你生硬地谈一个问题,就是没有想到需要说的内容而乱讲,这样两人脱口而出事后后悔的事情的几率就越大。Piver说。